PDF Versions of Standards for Human Stem Cell Use in Research

This document identifies quality standards and outlines basic core principles for the laboratory use of both tissue and pluripotent human stem cells and the in vitro model systems that rely on them. Overall, the emphasis of this document is creating a set of recommendations that, when taken together, establish the minimum characterization and reporting criteria for scientists, students, and technicians in basic research laboratories working with human stem cells.

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Cover page of a document titled 'Standards for Human Stem Cell Use in Research' published in June 2023 by the International Society for Stem Cell Research, supported by Burroughs Wellcome Fund, Doris Duke Charitable Foundation, and SFARI.

Reporting Practices Checklist

This document identifies quality standards and outlines basic core principles for the laboratory use of both tissue and pluripotent human stem cells and the in vitro model systems that rely on them. Overall, the emphasis of this document is creating a set of recommendations that, when taken together, establish the minimum characterization and reporting criteria for scientists, students, and technicians in basic research laboratories working with human stem cells.

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Page from a scientific report titled 'Reporting Practices for Publishing Results with Human Pluripotent and Tissue Stem Cells,' including sections on metadata, culture details, and basic characterization, with references and placeholders for manuscript information, and ISSCR branding at the bottom.

Overview

Overview
Section 1: Basic Characterization

Section 1: Basic Characterization
Section 2: Pluripotency and the Undifferentiated State

Section 2: Pluripotency and the Undifferentiated State
Section 3: Genomic Characterization

Section 3: Genomic Characterization
Section 4: Stem Cell-based Model Systems

Section 4: Stem Cell-based Model Systems
Section 5: Reporting

Section 5: Reporting
Acknowledgements

Acknowledgements
Appendix 1: Recommended Standard Characterization of Stem Cells

Appendix 1: Recommended Standard Characterization of Stem Cells
Appendix 2: Nomenclature Criteria

Appendix 2: Nomenclature Criteria
Appendix 3: Cell Culture Hygiene Practices

Appendix 3: Cell Culture Hygiene Practices
Appendix 4: Markers for the Identification of Undifferentiated hPSCs and Monitoring Multi-Lineage Di

Appendix 4: Markers for the Identification of Undifferentiated hPSCs and Monitoring Multi-Lineage Di
Appendix 5: Assessment of Methods for Genetic Analysis

Appendix 5: Assessment of Methods for Genetic Analysis
Appendix 6: Reporting Practices for Publishing Results with Human Pluripotent and Tissue Stem Cells

Appendix 6: Reporting Practices for Publishing Results with Human Pluripotent and Tissue Stem Cells
References

References

Steering Committee and Working Groups

  • Co-Chair - Tenneille Ludwig, PhD
    WiCell Research Institute, USA

    Co-Chair - Peter Andrews, DPhil
    The University of Sheffield, UK

    Ivana Barbaric, DPhil
    The University of Sheffield, UK

    Nissim Benvenisty, PhD
    Hebrew University, Israel

    Madeline Lancaster, PhD
    MRC Laboratory of Molecular Biology, UK

    Christine Mummery, PhD
    Leiden University Medical Center, Netherlands

    Martin Pera, PhD
    The Jackson Laboratory, USA

    Yoji Sato, PhD
    National Institute of Health Sciences, Japan

    Glyn Stacey, PhD
    International Stem Cell Banking Initiative, UK

    Christine A. Wells, DPhil
    University of Melbourne, Australia

    Tongbiao Zhao, PhD
    Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China

  • Co-Chair - Glyn Stacey, PhD
    International Stem Cell Banking Initiative, UK

    Co-Chair - Tenneille Ludwig. PhD
    WiCell Research Institute, USA

    Jeremy Crook, PhD
    The University of Sydney, Chris O’Brien Lifehouse, The University of Wollongong, Australia

    Laurence Daheron, PhD
    Harvard Stem Cell Institute, USA

    Jon Draper, PhD
    Stem Cell Network, Canada

    Lyn Healy, PhD
    Francis Crick Institute, UK

    Andreas Kurtz, PhD
    Fraunhofer Institute for Biomedical Engineering, Berlin Institute of Health at Charité, Germany

  • Co-Chair - Peter Andrews, DPhil
    The University of Sheffield, UK

    Co-Chair - Martin Pera, PhD
    The Jackson Laboratory, USA

    Lyn Healy, PhD
    Francis Crick Institute, UK

    Maneesha Inamdar, PhD
    Jawaharlal Nehru Centre for Advanced Scientific Research, Institute for Stem Cell Science and Regenerative Medicine, India

    Tenneille Ludwig, PhD
    WiCell Research Institute, USA

    Noriko Shimasaki, MD, PhD
    Center for iPS Research and Application, Kyoto, Kyoto Prefectural University of Medicine, Nagoya University, Japan, National University of Singapore, Japan

    Austin Smith, PhD
    University of Exeter, UK

  • Co-Chair - Ivana Barbaric, DPhil
    The University of Sheffield, UK

    Tenneille Ludwig, PhD
    WiCell Research Institute, USA

    Peter Andrews, DPhil
    The University of Sheffield, UK

    Nissim Benvenisty, PhD
    Hebrew University, Israel

    Martin Pera, PhD
    The Jackson Laboratory, USA

    Yoji Sato, PhD
    National Institute of Health Sciences, Japan

    Claudia Spits, PhD
    Vrije Universiteit Brussel, Belgium

    Tongbiao Zhao, PhD
    Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine, China

  • Co-Chair - Madeline Lancaster, PhD
    MRC Laboratory of Molecular Biology, UK

    Co-Chair - Christine Mummery, PhD
    Leiden University Medical Center, Netherlands

    Anita Bhattacharyya, PhD
    University of Wisconsin-Madison, USA

    Jeremy Crook, PhD
    The University of Sydney, Chris O’Brien Lifehouse, The University of Wollongong, Australia

    Meritxell Huch, PhD
    Max Planck Institute of Molecular Cell Biology and Genetics, and Center for Systems Biology, Dresden, Germany

    Kim Jensen, PhD
    Novo Nordisk Foundation Center for Stem Cell Medicine, University of Copenhagen, Denmark

    Prisca Liberali, PhD
    Friedrich Miescher Institute for Biomedical Research, Switzerland

    Matthias Lutolf, PhD
    Swiss Federal Institute of Technology (EPFL), Switzerland

    Jihwan Song, DPhil
    CHA University, and iPS Bio, Inc., Korea

    Christine A. Wells, PhD
    University of Melbourne, Australia

    Tongbiao Zhao
    Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine, China

The ISSCR Standards Initiative is made possible through contributions by:

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Doris Duke Foundation logo with a stylized letter D on the left and the foundation's name on the right.
Logo of SFARI, Simons Foundation Autism Research Initiative, with text and design elements.

The ISSCR's Standards for Human Stem Cell Use in Research are strictly copyrighted by the society. No part of this document may be produced in any form without written permission of The International Society for Stem Cell Research. Contact isscr@isscr.org for more information.

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