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Organizer Description: This workshop provides hands-on and theoretical training in gene-editing technologies for human pluripotent stem cells. It will cover the complete gene editing workflow, including:
• Project design
• Transfection of human pluripotent stem cells (hPSCs)
• Analysis of edited cell pools
• Clonal isolation
• Picking, passaging, and freezing clones in 96-well plates
• Screening and genotype confirmation
• Quality control of final clones