PhD Course: Induced Pluripotent Stem Cells, Gene-editing and Brain Cell Models

Monday, September 22, 2025 2:00 AM
Friday, September 26, 2025 9:00 AM

Frederiksberg Campus, Grønnegårdsvej 7 21 Bülowsvej Frederiksberg, 1870 Denmark (map)

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Organizer Description:
This course is free of charge for PhD students at Danish universities (except Copenhagen Business School), and for PhD Students from NorDoc member faculties. All other participants must pay the course fee.

Anyone can apply for the course, but if you are not a PhD student at a Danish university, you will be placed on the waiting list until enrollment deadline. This also applies to PhD students from NorDoc member faculties. After the enrollment deadline, available seats will be allocated to applicants on the waiting list.

Learning objectives
A student who has met the objectives of the course will be able to:

Skills:
• Freeze and thaw human induced pluripotent stem cells
• Maintain and expand human induced pluripotent stem cells
• Generate iPSC via electroporation of episomal plasmids
• Differentiate human induced pluripotent stem cells into astrocytes, microglia and cortical neurons
• Generate 3D cultures
• Analyze induced pluripotent stem cell-derived neural cells using different molecular methodologies
• Design CRISPRs guides and templates for gene-editing of pluripotent stem cells
• Perform nucleofection based CRISPR-Cas9 gene editing

Knowledge:
• Acquire a detailed understanding of the derivation of pluripotent stem cells
• Acquire a detailed theoretical understanding of the states of pluripotency
• Acquire a detailed understanding of early neural developmental processes and molecular cues that govern neural patterning
• Acquire a detailed understanding of the latest protocols for differentiation of iPSC into neural subtypes, astrocytes and microglia
• Gain detailed knowledge on state-of-the-art methods for culturing pluripotent stem cells
• Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into neural cell types (with focus on neurons, astrocytes) in 2D and 3D
• Gain detailed knowledge on the latest methods for differentiating pluripotent stem cells into microglia
• Gain expertise on the use and application of CRISPR guides in gene-editing of pluripotent stem cells