Standards for Human Stem Cell Use in Research

  • This document identifies quality standards and outlines basic core principles for the laboratory use of both tissue and pluripotent human stem cells and the in vitro model systems that rely on them. Overall, the emphasis of this document is creating a set of recommendations that, when taken together, establish the minimum characterization and reporting criteria for scientists, students, and technicians in basic research laboratories working with human stem cells.

  • Jump to the Overview section of the Standards Document. Learn more.

  • This checklist is intended to help scientists, reviewers, and editors prepare and assess manuscripts for inclusion of critical details relevant to work with pluripotent stem cells (PSCs) and tissue stem cells (TSCs) with the goal of increasing the rigor and reproducibility of research through reporting. It is essential that any published paper includes detailed information on the following parameters to increase the transparency of the experimental details and ensure that the published results are reproducible. Learn more.

Assess the rigor and reproducibility of your human pluripotent stem cell research. Download our fillable checklist PDF.

Overview

Overview
Section 1: Basic Characterization

Section 1: Basic Characterization
Section 2: Pluripotency and the Undifferentiated State

Section 2: Pluripotency and the Undifferentiated State
Section 3: Genomic Characterization

Section 3: Genomic Characterization
Section 4: Stem Cell-based Model Systems

Section 4: Stem Cell-based Model Systems
Section 5: Reporting

Section 5: Reporting
Acknowledgements

Acknowledgements
Appendix 1: Recommended Standard Characterization of Stem Cells

Appendix 1: Recommended Standard Characterization of Stem Cells
Appendix 2: Nomenclature Criteria

Appendix 2: Nomenclature Criteria
Appendix 3: Cell Culture Hygiene Practices

Appendix 3: Cell Culture Hygiene Practices
Appendix 4: Markers for the Identification of Undifferentiated hPSCs and Monitoring Multi-Lineage Di

Appendix 4: Markers for the Identification of Undifferentiated hPSCs and Monitoring Multi-Lineage Di
Appendix 5: Assessment of Methods for Genetic Analysis

Appendix 5: Assessment of Methods for Genetic Analysis
Appendix 6: Reporting Practices for Publishing Results with Human Pluripotent and Tissue Stem Cells

Appendix 6: Reporting Practices for Publishing Results with Human Pluripotent and Tissue Stem Cells
References

References

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The ISSCR's Standards for Human Stem Cell Use in Research are strictly copyrighted by the society. No part of this document may be produced in any form without written permission of The International Society for Stem Cell Research. Contact isscr@isscr.org for more information.

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